Developing Client Self-Advocacy in Occupational Therapy: Are We Practicing What We Preach?

Background: Developing client self-advocacy is in occupational therapy's (OT) scope of practice; however, there is limited understanding of if, or how, occupational therapists learn about self-advocacy interventions as well as implement self-advocacy into clinical practice. Objective: This study sought to identify if and how therapists learn about self-advocacy intervention approaches and identify if and how therapists implement self-advocacy into their work with clients. Method: A survey was distributed via email to academic and professional listservs in the United States, and data were collected using REDCap survey software. Descriptive statistics were analyzed data using REDCap/SPSS. Comparative statistics, Kruskal-Wallis's tests, Chi-square tests for independence, and Pearson's correlation tests analyzed differences across groups of respondents. Results: Practicing and licensed occupational therapists (n = 138) across the United States completed the survey. Findings indicate a majority (59.5%) of occupational therapists not learning strategies for addressing or developing client self-advocacy. Of significance, 21.7% of participants had never been exposed to concepts of client self-advocacy in academic or clinical education. Practitioners who did address self-advocacy did so indirectly through teaching-related skills (76.6%). Conclusion: Many clients of OT will need self-advocacy skills in order to address issues of exclusion and discrimination that prohibit full participation in society. Occupational therapists must prioritize incorporating client self-advocacy into curricula and clinical practice.

Microplastics and plastisphere at surface waters in the Southwestern Caribbean sea.

Pollution generated by plastic waste has brought an environmental problem characterized by the omnipresence of smaller pieces of this material known as microplastics (MP). This issue was addresses by collecting samples with 250 µm pore size nets in two marine-coastal sectors of Southwestern Caribbean Sea during two contrasting seasons. Higher concentrations were found in rainy season than in dry season, reaching respectively 1.72 MP/m3 and 0.22 MP/m3. Within each sector, there were differences caused firstly by localities of higher concentrations of semi-closed water bodies localities during rainy season (Ciénaga Grande de Santa Marta and La Caimanera marsh), and secondly by lower concentrations of localities with less influenced of flow rates during dry season (Salamanca and Isla Fuerte). Moreover, the lowest concentration in dry season corresponding to La Caimanera marsh reflects how the community environmental management might decrease MP pollution. In both sectors and seasons, the particles of 0.3 mm (0.3-1.4 mm) size class dominated over those of 1.4 mm (1.4-5.0 mm) (reaching each respectively 1.33 MP/m3 and 0.39 MP/m3), with a dominance of fibers, except in the rainy season in Magdalena, where they were films. Using the FTIR technique, polypropylene was identified as the most abundant polymer in both sectors. The composition of the assemblage of microorganisms attached to microplastics presented higher richness and differed from that of free-living planktonic microbes. The most abundant members of the plastisphere were proteobacteria whose major representation was the pathogenic genus Vibrio, while the cyanobacteria dominated in seawater samples.

Effect of low dose Semaglutide in people with Type 1 Diabetes and excess weight.

Obesity is a prevalent problem in people living with T1D (PwT1D), and it has been linked to cardiovascular disease in this population. The use of low dose weekly Semaglutide (0,5 mg) was evaluated in a cohort of PwT1D and excess weight.

Assessment of diaphragmatic function through surface electromyography in healthy individuais. A cross-section observational study

Introduction: Diaphragmatic surface electromyography is a procedure designed to assess the diaphragm. The physiological values of the electrical activity may have potential use in rehabilitation, sports training, ventilatory support withdrawal in critical care units and follow-up of respiratory disease. Objective: To assess and describe the diaphragmatic function through surface electromyography in a population of individuals during spontaneous and forced breathing. Methods: Observational, exploratory cross-sectional study including subjects with no comorbidities. Diaphragmatic surface EMG was performed measuring the mean quadratic root during tidal volume and vital capacity breathing. The body composition of the participants was also assessed. Results: 28 males and 22 females were included in the study. The mean quadratic root of the tidal volume for two minutes was 13.94 nV for females and 13.31 nV for males. The vital capacity was 23.24 nV for males and 22.4 nV for females. A correlation was identified between the mean quadratic root, weight, and body surface. Conclusions: Mean quadratic root values of tidal volume in two minutes in healthy females and males have been documented. The mean quadratic root values are correlated with the physiological and functional characteristics of the participants.

Introducción: La electromiografía de superficie diafragmática es un procedimiento para la evaluación diafragmática. Los valores fisiológicos de la actividad eléctrica tendrían aplicaciones potenciales en rehabilitación, entrenamiento deportivo, en el retiro ventilatorio en unidades de cuidado crítico y en el seguimiento a patologías respiratorias. Objetivo: Evaluar y describir la función diafragmática a través de electromiografía de superficie diafragmática en una población de sujetos durante la respiración espontánea y la respiración forzada. Métodos: Estudio observacional exploratorio de corte transversal en el que se incluyeron sujetos sin comorbilidades. Se realizó electromiografía de superficie diafragmática midiendo la raíz cuadrática media durante respiraciones de volumen corriente y capacidad vital. Adicionalmente, se valoró la composición corporal de los participantes. Resultados: Se incluyeron 28 hombres y 22 mujeres. La raíz cuadrática media de volumen corriente por dos minutos fue de 13,94 µV para mujeres y 13,31 µV para hombres, mientras que la capacidad vital fue 23,24 µV para hombres y 22,4 µV para mujeres. Se encontró una correlación entre la raíz cuadrática media, el peso y la superficie corporal. Conclusiones: Se han documentado los valores de la raíz cuadrática media de volumen corriente por dos minutos en mujeres y hombres sanos. Los valores de la raíz cuadrática media se correlacionan con características fisiológicas y funcionales de los participantes.

Factores pronósticos que influyen en los resultados de tratamiento de la periimplantitis: Una revisión sistemática

Objetivo: Esta revisión sistemática tiene como objetivo identificar los factores pronósticos y/o determinantes del éxito del tratamiento de la periimplantitis. Materiales y métodos: Se realizó una búsqueda bibliográfica estructurada y exhaustiva para identificar referencias relevantes en las bases de datos MEDLINE (PubMed), Scielo y Springerlik. La estrategia de búsqueda se realizó combinando los términos periimplantitis con factores pronósticos y / o determinantes del éxito del tratamiento como MeSH y texto libre combinando con los operadores booleanos AND y OR. Se utilizó la clasificación de SIGN (Scottish Intercollegiate Guidelines Network) para analizar el nivel de evidencia. Resultados: De los 239 artículos revisados, 17 cumplieron con los criterios de selección para el análisis cualitativo de la evidencia. Conclusiones: El tipo de implante (macro y microgeometría) y la morfología del defecto, los cuales dificulta el acceso a las superficies contaminadas, son factores que influyen en el resultado del tratamiento de la periimplantitis. No hay evidencia sobre el método de descontaminación más eficaz. Las condiciones generales como la diabetes mellitus y la presencia de enfermedad periodontal activa afectan el resultado del tratamiento, así como la frecuencia de un programa de mantención de higiene profesional postquirúrgica, la cual mejora la sobrevida del implante.

Objective: This systematic review aimed at identifying prognostic factors influencing periimplantitis treatment outcomes. Materials and methods: A structured and comprehensive literature search was performed to identify relevant references from MEDLINE (PubMed), Scielo and Springerlik databases. The search strategy was carried out combining the search terms periimplantitis with prognostic factors and/or determinants of treatment success as MeSH and free text combining with the boolean logical operators AND and OR. The SIGN (Scottish Intercollegiate Guidelines Network) was used to analyze the level of evidence. Results: From 239 articles reviewed, 18 fulfilled the selection criteria for the qualitative analysis of the evidence. Conclusions: The type of implant (macro and microgeometry) and the defect morphology, which make access to contaminated surfaces difficult, are factors that influence the outcome of peri-implantitis treatment. There is no evidence on the most effective decontamination method. General conditions such as diabetes mellitus and the presence of active periodontal disease affect the outcome of the treatment. as Also, the frequency of a post-surgical professional hygiene maintenance program improves the dental implant survival post-treatment.

Variation in phytoplankton diversity during phycoremediation in a polluted Colombian Caribbean swamp.

Phytoplankton composition and abundance are considered among the bioindicators of variations in water quality, due to its sensitivity and rapid responses to changes in environmental parameters. The selection and scaling of the Microalgal Predominant Microbial Consortium (MPMC) were based on live samples collected from the Santiago Apóstol Swamp (SAS) and the Arroyo Grande de Corozal (AGC). The inoculum was scaled in a phycoculture plant, and the inoculation process was performed in the AGC that flows into the SAS. The phytoplankton community monitoring was performed from May 2019 to September 2021. In the process, a total of 1,652,258 gallons were inoculated. Precisely, 103 and 124 species were found in the AGC and SAS, respectively. By evaluating the physical, chemical, and microbiological variables in SAS in a multitemporal way based on the inoculation of the MPMC, it is possible to identify the variables that presented the greatest reduction. The density of SAS presented associations with dissolved oxygen, thermotolerant coliforms, Enterococci, pH, phosphorus, nitrates, speed, and Secchi. The AGC and SAS presented high trophic levels (eutrophication). This contamination by organic matter is probably due to the discharges of the domestic tributaries. The diversity of microalgae and cyanobacteria found in this study allows us to know the anthropogenic impact. The density of microalgae showed the positive impact of the treatment with MCPM, where there was a decrease in the species that denote organic contamination. The phytoremediation treatment was effective in terms of the changes observed in the physicochemical variables, and these changes were directly due to the efficiency of the treatment and not the natural behavior of the water sources in the region.

El impacto en la calidad de vida, la depresión y el manejo analgésico se asocian al sufrimiento en pacientes con fibromialgia. Un estudio transversal / Impact on quality of life, depression and analgesic use, are associated with suffering in fibromyalgia patients. A cross-sectional study

Introducción: La fibromialgia (FM) es un trastorno complejo que genera impacto en la salud pública. Son escasos los estudios que determinan el sufrimiento en FM, pues el abordaje se enfoca en el dolor. Objetivo: Estimar la prevalencia de sufrimiento en los pacientes con FM y su asociación con el impacto en calidad de vida, malestar emocional, soledad, eventos vitales y aspectos clínicos y sociodemográficos en una institución de salud privada de Medellín (Colombia). Método: Se realizó un estudio transversal, en una institución especializada en manejo de dolor crónico. Se evaluó el grado de sufrimiento (PRISM), nivel de dolor (EVA), impacto de la FM (FIQR), soledad (UCLA), ansiedad y depresión (HADS), eventos vitales estresantes (CSV) y las características sociodemográficas y clínicas. Se planteó un modelo explicativo con una regresión logística. Resultados: El 55,5 % IC95 (50,0-61,1) presentó sufrimiento intenso. Se obtuvo un modelo explicativo, donde el impacto en la calidad de vida, la depresión y la toma de analgésicos fueron los factores más relevantes en el sufrimiento intenso. Discusión: Los pacientes con FM manifiestan un sufrimiento importante que debe ser atendido por el personal de salud. Si bien el dolor es un elemento relevante, existen otros factores a los que debe prestarse atención, como el impacto que causa la enfermedad en la persona y la depresión asociada.(AU)

Introduction: Fibromyalgia (FM) is a complex disorder with significant impact on public health. Few studies have explored suffering in FM, since its approach focuses on pain management. Objective: To estimate the prevalence of suffering in patients with FM and its association with impact on quality of life, emotional distress, loneliness, vital stress and clinical and sociodemographic factors in a private healthcare institution in Medellín (Colombia). Method: A cross-sectional study was conducted in an institution specialized in chronic pain management. The degree of suffering (PRISM), level of pain (EVA), impact of FM (FIQR), loneliness (UCLA), anxiety and depression (HADS), stressful life events (CSV) and sociodemographic and clinical characteristics were evaluated. An explanatory model was proposed using logistic regression. Results: 55.5 % IC95 (50,0-61,1) presented intense suffering. An explanatory model was obtained, where impact, depression and analgesic intake were the most relevant factors in intense suffering.Discussion: Patients with FM manifest an important suffering that should be attended by health personnel. Although pain is a relevant element, there are other factors to which attention should be paid, such as the impact of the disease on the person and the associated depression.(AU)

Estudio preliminar para la validación de la versión chilena del Vocal Tract Discomfort-VTD en población docente / Validation of the chilean version of the Vocal Tract Discomfort-VTD on teachers: a preliminary study

Debido a la carga vocal a la que están expuestos los profesionales de la voz, como los profesores, son frecuentes los trastornos de voz y mal uso vocal en este grupo, lo que hace necesario poder contar con instrumentos de pesquisa rápidos y sencillos para un diagnóstico precoz de sintomatología vocal. El objetivo del presente estudio es realizar una adaptación y validación preliminar de la pauta "Vocal Tract Discomfort Scale (VTD)" al idioma español chileno en profesores. Se comenzó con la traducción al español del instrumento original por parte de dos expertos nativos y luego la retro-traducción por dos expertos en idioma inglés para determinar concordancias y discrepancias. Luego se continuó con la validación de apariencia y contenido realizada por valoración de grupo de expertos compuesto por dos metodólogos y cinco fonoaudiólogos con experiencia en el área vocal. La validez de criterio se obtuvo mediante la correlación entre la escala en estudio y el Voice Handicap Index en su versión validada al español como prueba gold-standard, para lo cual se aplicó en una muestra de 31 docentes. La validación de la escala VTD es fiable y deja a disposición de los fonoaudiólogos una herramienta sencilla y comprensible para el entendimiento del impacto generado en personas con disfonía, particularmente, en profesionales de la voz como los profesores.

Due to the vocal load to which voice professionals such as teachers are exposed, voice disorders and vocal misuse are frequently found in this group. This makes it necessary to have quick and simple assessment instruments that allow early detection of vocal symptoms. The objective of this study was to adapt the "Vocal Tract Discomfort Scale (VTD)" to Chilean Spanish and carry out a preliminary validation of this protocol on teachers. First, the original instrument was translated toSpanish by two native speakers, and then back-translated by two English experts, in order to determine the presence of agreements and discrepancies. Subsequently, a group of experts made up of two methodologists and five speech therapists with experience in the voice field performed an appearance and content validation. Criterion validity was obtained through the correlation between the Vocal Tract Discomfort Scale and the Spanish-validated version of the Voice Handicap Index as a gold-standard test, for which the first was appliedto a sample of 31 teachers. The validation of the VTD scale is reliable and provides speech therapists with a simple and understandable tool that allows them to comprehend the impact of dysphonia on people, particularly on voice professionals such as teachers.

Comprehensive assessment of respiratory function, a step towards early weaning from the ventilator.

Methods for assessing diaphragmatic function can be useful in determining the functional status of the respiratory system and can contribute to determining an individual's prognosis, depending on their pathology. They can also be a useful tool for making objective decisions regarding mechanical ventilation weaning and extubation. Esophageal and transdiaphragmatic pressure measurement, diaphragm ultrasound, diaphragmatic excursion, surface electromyography (sEMG) and some serum biomarkers are of increasing interest and use in clinical and intensive care settings to offer a more objective process for withdrawing mechanical ventilation; especially in the situation that we are experiencing with the increased demand for mechanical ventilation to treat patients with Covid-19-associated viral pneumonia. In this literature review, we updated the clinical and physiological indicators with more evidence to improve ventilator withdrawal techniques. We concluded that, to ensure successful extubation in a way that is useful, cost-effective, practical for health personnel and non-invasive for the patient, further studies of novel techniques such as surface electromyography should be implemented.

Changes in energy metabolism induced by fluoride: Insights from inside the mitochondria.

The mechanisms involved in changes in energy metabolism caused by excessive exposure to fluoride (F) are not completely understood. The present study employed proteomic tools to investigate the molecular mechanisms underlying the dose- and time-dependency of the effects of F in the rat liver mitochondria. Thirty-six male Wistar rats received water containing 0, 15 or 50 mgF/L (as NaF) for 20 or 60 days. Rat liver mitochondria were isolated and the proteome profiles were examined using label-free quantitative nLC-MS/MS. PLGS software was used to detect changes in protein expression among the different groups. The bioinformatics analysis was done using the software CYTOSCAPE® 3.0.7 (Java®) with the aid of ClueGo plugin. The dose of 15 mgF/L, when administered for 20 days, reduced glycolysis, which was counterbalanced by an increase in other energetic pathways. At 60 days, however, an increase in all energy pathways was observed. On the other hand, the dose of 50 mgF/L, when administered for 20 days, reduced the enzymes involved in all energetic pathways, indicating a lower rate of energy production, with less generation of ROS and consequent reduction of antioxidant enzymes. However, when the 50 mgF/L dose was administered for 60 days, an increase in energy metabolism was seen but in general no changes were observed in the antioxidant enzymes. Except for the group treated with 50 mgF/L for 20 days, all the other groups had alterations in proteins in attempt to maintain calcium homeostasis and avoid apoptosis. The results suggest that the organism seems to adapt to the effects of F over time, activating pathways to reduce the toxicity of this ion. Ultimately, our findings corroborate the safety of the use of fluoride for caries control.

Centro de presión y patrones de actividad muscular durante la ejecución de una sentadilla monopodal isométrica realizada sobre una superficie esponjosa en mujeres jóvenes sanas / Center of pressure and patterns of muscular activity during the execution of an isometric single leg squat performed on a spongy surface in healthy young women / Centro de pressão e padrões de atividade muscular durante a execução de um agachamento isométrico uni podal realizado em uma superfície esponjosa por mulheres jovens saudáveis

Objetivo: Comparar la ejecución del ejercicio de sentadilla monopodal isométrica, en una superficie rígida versus una superficie esponjosa, en relación al centro de presión y los patrones de activación de la musculatura de la extremidad inferior dominante y la columna, en mujeres jóvenes sanas. Método: 12 mujeres sanas, realizaron la sentadilla monopodal isométrica en una superficie rígida (control) y una superficie esponjosa. Un posturógrafo registró el área y velocidad de desplazamiento del centro de presión. Un electromiógrafo de superficie evaluó la actividad muscular de multífido, glúteo mayor, glúteo medio, vasto medial y bíceps femoral de la pierna de apoyo. Resultados: El área y la velocidad de desplazamiento del centro de presión aumentó (p<0.05 y p<0.01 respectivamente) en superficie esponjosa. El bíceps femoral aumenta su actividad muscular en superficie esponjosa (p<0.05). Existe correlación entre la mayor actividad muscular del bíceps femoral y el área y la velocidad del centro de presión (p<0.01). Conclusión: La sentadilla monopodal isométrica realizada sobre una superficie esponjosa, produce una mayor perturbación del control postural y una mayor actividad del bíceps femoral en mujeres jóvenes sanas. El aumento de actividad de este músculo se relaciona directamente con el desplazamiento del centro de presión

Objective: To compare the performance of the isometric single leg squat exercise on a rigid surface versus a spongy surface in relation to the center of pressure and activation patterns of the muscles of the dominant lower limb and spine in healthy young women. Method: 12 healthy women performed the isometric single leg squat on a rigid surface (control) and a spongy surface. A posturograph recorded the area and velocity of displacement of the pressure center. A surface electromyograph evaluated the muscular activity of multifidus, gluteus maximus, gluteus medius, vastus medialis and biceps femoris of the supporting leg. Results: The area and the speed of displacement of the center of pressure increased (p <0.05 and p <0.01 respectively) in spongy surface. Femoral biceps increase its muscular activity on the spongy surface (p <0.05). There is a correlation between the greater muscular activity of the biceps femoris and the area and the velocity of the pressure center (p <0.01). Conclusion: Isometric single leg squat performed on a spongy surface, produces a greater disturbance of postural control and greater activity of the biceps femoris in healthy young women. The increased activity of this muscle is directly related to the displacement of the pressure center

Objetivo: Comparar o desempenho no agachamento isométrico uni podal em superfície rígida versus superfície esponjosa em relação ao centro de pressão e padrões de ativação dos músculos do membro inferior dominante e coluna vertebral em mulheres jovens saudáveis. Método: 12 mulheres saudáveis realizaram o agachamento isométrico uni podal em uma superfície rígida (controle) e uma superfície esponjosa. Um posturógrafo registrou a área e a velocidade de deslocamento do centro de pressão. Um eletromiógrafo de superfície avaliou a atividade muscular de multifidus, glúteo máximo, glúteo médio, vasto medial e bíceps femoral da perna de apoio. Resultados: A área e a velocidade de deslocamento do centro de pressão aumentaram (p <0.05 e p <0.01, respectivamente) na superfície esponjosa. O bíceps femoral aumenta sua atividade muscular na superfície esponjosa (p <0.05). Existe uma correlação entre a maior atividade muscular do bíceps femoral e a área e a velocidade do centro de pressão (p <0.01). Conclusão: Agachamento isométrico uni podal realizado em uma superfície esponjosa, produz uma maior perturbação do controle postural e uma maior atividade do bíceps femoral em mulheres jovens saudáveis. O aumento da atividade desse músculo está diretamente relacionado ao deslocamento do centro de pressão

Comparative transcriptome analysis reveals different strategies for degradation of steam-exploded sugarcane bagasse by Aspergillus niger and Trichoderma reesei.

BACKGROUND: Second generation (2G) ethanol is produced by breaking down lignocellulosic biomass into fermentable sugars. In Brazil, sugarcane bagasse has been proposed as the lignocellulosic residue for this biofuel production. The enzymatic cocktails for the degradation of biomass-derived polysaccharides are mostly produced by fungi, such as Aspergillus niger and Trichoderma reesei. However, it is not yet fully understood how these microorganisms degrade plant biomass. In order to identify transcriptomic changes during steam-exploded bagasse (SEB) breakdown, we conducted a RNA-seq comparative transcriptome profiling of both fungi growing on SEB as carbon source. RESULTS: Particular attention was focused on CAZymes, sugar transporters, transcription factors (TFs) and other proteins related to lignocellulose degradation. Although genes coding for the main enzymes involved in biomass deconstruction were expressed by both fungal strains since the beginning of the growth in SEB, significant differences were found in their expression profiles. The expression of these enzymes is mainly regulated at the transcription level, and A. niger and T. reesei also showed differences in TFs content and in their expression. Several sugar transporters that were induced in both fungal strains could be new players on biomass degradation besides their role in sugar uptake. Interestingly, our findings revealed that in both strains several genes that code for proteins of unknown function and pro-oxidant, antioxidant, and detoxification enzymes were induced during growth in SEB as carbon source, but their specific roles on lignocellulose degradation remain to be elucidated. CONCLUSIONS: This is the first report of a time-course experiment monitoring the degradation of pretreated bagasse by two important fungi using the RNA-seq technology. It was possible to identify a set of genes that might be applied in several biotechnology fields. The data suggest that these two microorganisms employ different strategies for biomass breakdown. This knowledge can be exploited for the rational design of enzymatic cocktails and 2G ethanol production improvement.

Comparative Secretome Analysis of Trichoderma reesei and Aspergillus niger during Growth on Sugarcane Biomass.

BACKGROUND: Our dependence on fossil fuel sources and concern about the environment has generated a worldwide interest in establishing new sources of fuel and energy. Thus, the use of ethanol as a fuel is advantageous because it is an inexhaustible energy source and has minimal environmental impact. Currently, Brazil is the world's second largest producer of ethanol, which is produced from sugarcane juice fermentation. However, several studies suggest that Brazil could double its production per hectare by using sugarcane bagasse and straw, known as second-generation (2G) bioethanol. Nevertheless, the use of this biomass presents a challenge because the plant cell wall structure, which is composed of complex sugars (cellulose and hemicelluloses), must be broken down into fermentable sugar, such as glucose and xylose. To achieve this goal, several types of hydrolytic enzymes are necessary, and these enzymes represent the majority of the cost associated with 2G bioethanol processing. Reducing the cost of the saccharification process can be achieved via a comprehensive understanding of the hydrolytic mechanisms and enzyme secretion of polysaccharide-hydrolyzing microorganisms. In many natural habitats, several microorganisms degrade lignocellulosic biomass through a set of enzymes that act synergistically. In this study, two fungal species, Aspergillus niger and Trichoderma reesei, were grown on sugarcane biomass with two levels of cell wall complexity, culm in natura and pretreated bagasse. The production of enzymes related to biomass degradation was monitored using secretome analyses after 6, 12 and 24 hours. Concurrently, we analyzed the sugars in the supernatant. RESULTS: Analyzing the concentration of monosaccharides in the supernatant, we observed that both species are able to disassemble the polysaccharides of sugarcane cell walls since 6 hours post-inoculation. The sugars from the polysaccharides such as arabinoxylan and ß-glucan (that compose the most external part of the cell wall in sugarcane) are likely the first to be released and assimilated by both species of fungi. At all time points tested, A. niger produced more enzymes (quantitatively and qualitatively) than T. reesei. However, the most important enzymes related to biomass degradation, including cellobiohydrolases, endoglucanases, ß-glucosidases, ß-xylosidases, endoxylanases, xyloglucanases, and α-arabinofuranosidases, were identified in both secretomes. We also noticed that the both fungi produce more enzymes when grown in culm as a single carbon source. CONCLUSION: Our work provides a detailed qualitative and semi-quantitative secretome analysis of A. niger and T. reesei grown on sugarcane biomass. Our data indicate that a combination of enzymes from both fungi is an interesting option to increase saccharification efficiency. In other words, these two fungal species might be combined for their usage in industrial processes.

Calidad de vida relacionada con la salud de niños con enfermedades crónicas alojados en un hospedaje integral en la Ciudad de Buenos Aires / Health-related quality of life in children with chronic conditions lodged at a comprehensive accommodation in the City of Buenos Aires

Introducción. El tratamiento de enfermedades crónicas suele requerir un entrenamiento profesional multidisciplinario. Existen alojamientos integrales adaptados a las necesidades de familias que deben permanecer por períodos prolongados lejos de su hogar. Objetivos. Evaluar el impacto de un programa de hospedaje integral sobre la calidad de vida relacionada con la salud de niños con patologías crónicas atendidos en un hospital de la Ciudad de Buenos Aires, en comparación con niños alojados en hospedajes tradicionales y con niños sanos. Analizar la concordancia entre la declaración de niños y cuidadores. Métodos. Estudio observacional transversal. Grupo 1: niños con trastornos crónicos hospedados en un alojamiento integral; Grupo 2: niños con trastornos crónicos hospedados en un alojamiento tradicional; Grupo 3: niños sanos. Se utilizó la versión argentina del cuestionario Kidscreen-52. El valor p < 0,05, tamaño de efecto (TE) > 0,20 y el coeficiente de correlación interclase (CCI) > 0,60 fueron considerados significativos. Resultados. 250 duplas niños/as-cuidadores (50 en cada grupo crónico y 150 controles sanos). Edad media: 12,6 años (rango de 8-18 años); 56%, varones. Niños del Grupo 1 manifestaron mejor estado de ánimo, relación parental, amigos y entorno escolar comparado con el Grupo 2 (p < 0,05, TE > 0,20). La concordancia entre la declaración de niños y cuidadores fue baja, principalmente en las dimensiones psicosociales (CCI < 0,29). Conclusiones. Los niños con trastornos crónicos pueden conservar, pese al desarraigo, una buena relación parental, lazos de amistad y apoyo social, y no ver discontinuada su escolaridad, en un hogar integral adaptado a sus necesidades durante su estadía en la Ciudad de Buenos Aires.

Introduction.The treatment of chronic diseases usually requires a multidisciplinary professional training.There are comprehensive accommodations that are adapted to the needs of the families that have to stay away from home for long periods. Objectives.To assess the impact of a comprehensive accommodation on the health-related quality of life of children with chronic conditions seen at a hospital of the City of Buenos Aires compared to children lodged at a traditional accommodation and to healthy children. To analyze the agreement between children's and caregivers' opinions. Metfeods.Cross-sectional, observational study. Group 1:children with chronic conditions lodged at a comprehensive accommodation. Group 2: children with chronic conditions lodged at a traditional accommodation. Group 3: healthy children. The Argentine version of the Kidscreen-52 questionnaire was used. A value of p < 0.05, the effect size (ES) > 0.20, and the intraclass correlation coefficient (ICC) > 0.60 were considered significant. Results.Two hundred and fifty children/caregiver dyads (50 in each chronic group and 150 healthy controls). Mean age: 12.6 years old (range: 8-18 years old), 56% were males. The children in Group 1 showed better moods and emotions, a better relationship with parents and peers, and a better school environment than those in Group 2 (p < 0.05, ES > 0.20). Agreement between children's and caregivers' opinion was low, especially in relation to psychosocial dimensions (ICC < 0.29). Conclusions.In spite of their uprooting, children with chronic conditions are capable of maintaining a good relationship with their parents, a good peer bond and social support, and do not need discontinue their education while lodged at a comprehensive accommodation adapted to their needs during their stay in the City of Buenos Aires.

Calidad de vida relacionada con la salud de niños con enfermedades crónicas alojados en un hospedaje integral en la Ciudad de Buenos Aires / Health-related quality of life in children with chronic conditions lodged at a comprehensive accommodation in the City of Buenos Aires

Introducción. El tratamiento de enfermedades crónicas suele requerir un entrenamiento profesional multidisciplinario. Existen alojamientos integrales adaptados a las necesidades de familias que deben permanecer por períodos prolongados lejos de su hogar. Objetivos. Evaluar el impacto de un programa de hospedaje integral sobre la calidad de vida relacionada con la salud de niños con patologías crónicas atendidos en un hospital de la Ciudad de Buenos Aires, en comparación con niños alojados en hospedajes tradicionales y con niños sanos. Analizar la concordancia entre la declaración de niños y cuidadores. Métodos. Estudio observacional transversal. Grupo 1: niños con trastornos crónicos hospedados en un alojamiento integral; Grupo 2: niños con trastornos crónicos hospedados en un alojamiento tradicional; Grupo 3: niños sanos. Se utilizó la versión argentina del cuestionario Kidscreen-52. El valor p < 0,05, tamaño de efecto (TE) > 0,20 y el coeficiente de correlación interclase (CCI) > 0,60 fueron considerados significativos. Resultados. 250 duplas niños/as-cuidadores (50 en cada grupo crónico y 150 controles sanos). Edad media: 12,6 años (rango de 8-18 años); 56%, varones. Niños del Grupo 1 manifestaron mejor estado de ánimo, relación parental, amigos y entorno escolar comparado con el Grupo 2 (p < 0,05, TE > 0,20). La concordancia entre la declaración de niños y cuidadores fue baja, principalmente en las dimensiones psicosociales (CCI < 0,29). Conclusiones. Los niños con trastornos crónicos pueden conservar, pese al desarraigo, una buena relación parental, lazos de amistad y apoyo social, y no ver discontinuada su escolaridad, en un hogar integral adaptado a sus necesidades durante su estadía en la Ciudad de Buenos Aires.(AU)

Introduction.The treatment of chronic diseases usually requires a multidisciplinary professional training.There are comprehensive accommodations that are adapted to the needs of the families that have to stay away from home for long periods. Objectives.To assess the impact of a comprehensive accommodation on the health-related quality of life of children with chronic conditions seen at a hospital of the City of Buenos Aires compared to children lodged at a traditional accommodation and to healthy children. To analyze the agreement between childrens and caregivers opinions. Metfeods.Cross-sectional, observational study. Group 1:children with chronic conditions lodged at a comprehensive accommodation. Group 2: children with chronic conditions lodged at a traditional accommodation. Group 3: healthy children. The Argentine version of the Kidscreen-52 questionnaire was used. A value of p < 0.05, the effect size (ES) > 0.20, and the intraclass correlation coefficient (ICC) > 0.60 were considered significant. Results.Two hundred and fifty children/caregiver dyads (50 in each chronic group and 150 healthy controls). Mean age: 12.6 years old (range: 8-18 years old), 56% were males. The children in Group 1 showed better moods and emotions, a better relationship with parents and peers, and a better school environment than those in Group 2 (p < 0.05, ES > 0.20). Agreement between childrens and caregivers opinion was low, especially in relation to psychosocial dimensions (ICC < 0.29). Conclusions.In spite of their uprooting, children with chronic conditions are capable of maintaining a good relationship with their parents, a good peer bond and social support, and do not need discontinue their education while lodged at a comprehensive accommodation adapted to their needs during their stay in the City of Buenos Aires.(AU)

Health-related quality of life in children with chronic conditions lodged at a comprehensive accommodation in the City of Buenos Aires.

INTRODUCTION: The treatment of chronic diseases usually requires a multidisciplinary professional training.There are comprehensive accommodations that are adapted to the needs of the families that have to stay away from home for long periods. OBJECTIVES: To assess the impact of a comprehensive accommodation on the health-related quality of life of children with chronic conditions seen at a hospital of the City of Buenos Aires compared to children lodged at a traditional accommodation and to healthy children. To analyze the agreement between children's and caregivers' opinions. Metfeods.Cross-sectional, observational study. Group 1: children with chronic conditions lodged at a comprehensive accommodation. Group 2: children with chronic conditions lodged at a traditional accommodation. Group 3: healthy children. The Argentine version of the Kidscreen-52 questionnaire was used. A value of p < 0.05, the effect size (ES) > 0.20, and the intraclass correlation coefficient (ICC) > 0.60 were considered significant. RESULTS: Two hundred and fifty children/caregiver dyads (50 in each chronic group and 150 healthy controls). Mean age: 12.6 years old (range: 8-18 years old), 56% were males. The children in Group 1 showed better moods and emotions, a better relationship with parents and peers, and a better school environment than those in Group 2 (p < 0.05, ES > 0.20). Agreement between children's and caregivers' opinion was low, especially in relation to psychosocial dimensions (ICC < 0.29). CONCLUSIONS: In spite of their uprooting, children with chronic conditions are capable of maintaining a good relationship with their parents, a good peer bond and social support, and do not need discontinue their education while lodged at a comprehensive accommodation adapted to their needs during their stay in the City of Buenos Aires.

Health-related quality of life in children with chronic conditions lodged at a comprehensive accommodation in the City of Buenos Aires. / Health-related quality of life in children with chronic conditions lodged at a comprehensive accommodation in the City of Buenos Aires.

INTRODUCTION: The treatment of chronic diseases usually requires a multidisciplinary professional training.There are comprehensive accommodations that are adapted to the needs of the families that have to stay away from home for long periods. OBJECTIVES: To assess the impact of a comprehensive accommodation on the health-related quality of life of children with chronic conditions seen at a hospital of the City of Buenos Aires compared to children lodged at a traditional accommodation and to healthy children. To analyze the agreement between childrens and caregivers opinions. Metfeods.Cross-sectional, observational study. Group 1: children with chronic conditions lodged at a comprehensive accommodation. Group 2: children with chronic conditions lodged at a traditional accommodation. Group 3: healthy children. The Argentine version of the Kidscreen-52 questionnaire was used. A value of p < 0.05, the effect size (ES) > 0.20, and the intraclass correlation coefficient (ICC) > 0.60 were considered significant. RESULTS: Two hundred and fifty children/caregiver dyads (50 in each chronic group and 150 healthy controls). Mean age: 12.6 years old (range: 8-18 years old), 56

were males. The children in Group 1 showed better moods and emotions, a better relationship with parents and peers, and a better school environment than those in Group 2 (p < 0.05, ES > 0.20). Agreement between childrens and caregivers opinion was low, especially in relation to psychosocial dimensions (ICC < 0.29). CONCLUSIONS: In spite of their uprooting, children with chronic conditions are capable of maintaining a good relationship with their parents, a good peer bond and social support, and do not need discontinue their education while lodged at a comprehensive accommodation adapted to their needs during their stay in the City of Buenos Aires.

Avaliação do efeito do óleo-resina de copaíba (Copaifera s) na proliferação celular in vitro / Effect of copaiba oleoresin (Copaifera sp) on the in vitro cellular proliferation

O óleo de copaíba é uma resina extraída pela perfuração do tronco de árvores do gênero Copaifera sp., usado como cicatrizante em várias regiões do Brasil. São comprovados seus efeitos antimicrobianos e antiinflamatórios, sem relatos de atividade sobre a proliferação celular. No presente trabalho foi observada a dinâmica da proliferação de células MDBK (Madin Darby Bovine Kidney) mantidas em meio de cultivo adicionado de diferentes concentrações do óleo de copaíba, utilizando-se como controle células mantidas em meio sem adição do oleoresina (grupo M) e células no meio com aplicação do solvente tween 80, na diluição 10-3 (grupo MT). Diluições decimais de 10-1 até 10-3 mostraram-se tóxicas e, portanto, os estudos de proliferação partiram da diluição 10-4 até 10-7. Observou-se que houve crescimento mais acelerado em todos os grupos adicionados do óleo-resina nas primeiras 24 horas, com destaque para a diluição 10-5, que teve sua taxa de proliferação 5,47 vezes maior que a do grupo M. Concluiu-se que o óleo-resina de copaíba se mostrou estimulante da multiplicação celular, o que pode ser um dos mecanismos de seu efeito positivo sobre a cicatrização, somado àqueles previamente comprovados na literatura.

Copaiba oleoresin is extracted from the trunk of Copaifera sp genus trees and used for treating wounds in several regions of Brazil. Its antimicrobial and anti-inflammatory effects have been proved, however there are no reports of activity on cell growth. Proliferation of MDBK (Madin Darby Bovine Kidney) cells was evaluated under the influence of different concentrations of Copaiba oleoresin. Control groups consisted of cells in medium without addition of oleoresin (M group) and cells in the medium with application of the solvent Tween 80 at dilution 10-3 (TM group). Decimal dilutions of 10-1 to 10-3 were shown to be toxic and, therefore, the proliferation studies were conducted from dilution 10-4 to 10-7. Cell growth was faster in all groups that received the Copaiba oleoresin dilutions in the first 24 hours, specially the 10-5 dilution group, which proliferation rate was 5,47 higher than that of M group. It was concluded that Copaiba oleoresin stimulates cell multiplication, which may be one mechanism of its positive effect on wound healing, in association with those previously known.

Quality of life in pediatric liver transplantation in a single-center in South America.

HRQOL in children after LT has not been systematically measured in transplant recipients from South American countries. The aim of this study was to determine the HRQOL using a validated measure for children. The CHQOL-PF50 was completed by the parents of 54 patients after the clinical assessment. Subscale mean scores were compared with both a normal population (n = 274) and a group of chronic illness patients with Juvenile Idiopathic Arthritis (n = 23). Compared with the normal population, LT recipients had lower subscales scores for general health perceptions, role/social emotional, mental health, and parental impact on time. Bodily pain was significantly lower in our study group. Both mean physical and psychosocial summary scores were lower compared to the normal population but similar to the JIA group. Within the LT population, gender, original diagnosis, type of immunosuppression, type of transplant and time elapsed since LT did not significantly influence any of the summary scores. Our study showed LT children's physical and psycho-social areas were lower compared with those of the general population. LT children had less limitations due to pain. Family functioning appeared normal.